Preparation of samples is a critical stage if you require to carry out successful chromatography. Nylon filters help to ensure proper elimination of particles and microorganisms in the sample preparation process. Filters provide consistent and reliable experimental results for a range of samples and applications. Syringe filters protect chromatography columns by preventing the accumulation of fine particles in the column, which may form premature blockages.


Filters provide high-quality filtration of your samples and have features including

  • Color coding for easy identification of membrane and pore size
  • Leur Lock inlet to provide a secure attachment to the syringe
  • An integral ring to enhance housing strength to prevent bursting and breaking
  • Support low hold-up volume for treatment of small sample volumes with less minimal loss
  • Borosilicate pre-filter glass to provide more filtrate volumes
  • Criteria for selecting a syringe filter membrane

There are a few guides you have to follow when choosing the appropriate layer for your application. You need to consider some of the following:

  • Compatibility of the membrane with the solvent
  • Layer compatibility with the procedure and sample type
  • Amount of particles which need removing from the sample
  • Volume of the sample
  • Types of filters
  • Nylon syringe filters

When preparing aqueous or organic samples, nylon syringe filters are the preferred choice. There are used in analytical applications because they have excellent flow rates and give high throughput loading. Because they have good flow characteristics including mechanical stability, nylon filters can meet stringent analytical requirements.

Polypropylene syringe filters

These have a hydrophilic polypropylene membrane which is easy to get wet with water. It does not need prior treatment with alcohols, and it’s compatible with a lot of biological samples. However, it’s not recommended to use them with high organic solvents, especially aromatic and chlorinated solvents.

PTFE syringe filters

PTFE membranes need pre-treatment with alcohol before being suitable for aqueous or high aqueous samples. You cannot use them directly with aqueous solutions. The membrane’s hydrophobic nature makes PTFE filters utilized as a moisture barrier in venting applications.

Hydrophilic PTFE filters

These filters have a modified hydrophilic layer to provide excellent flow rates and high loading capacities. These syringe filters are usable when filtering organic or aqueous solvent samples. They have good chemical resistance.

PVDF syringe filters

PVDF syringe filters possess a well-defined pore construction. It enables them to give appropriate retaining of particles without excessive blockage of pores. They are harmonious with aqueous and most organic solvents. PVDF filters are excellent all-purpose filters for HPLC and organic solvent sample clean up.

Regenerated cellulose syringe filters

These filters are suited for almost any laboratory procedure. They can handle HPLC sample preparation when handling dissolution sample testing. The regenerated cellulose has better chemical resistance in either aqueous or organic-based sample environments. Its very little biological-based binding coefficient is well suited for ample sample recoveries of biological-based assays.

With the above tips, your syringe filter search has been made simpler. Putting that in mind the next time you are preparing your samples might lead to accurate result results.